Researchers from the Department of Chemistry, Katarina Radman and Branimir Bertoša, together with recent researcher from the Department of Chemistry Zoe Jelić Matošević, in collaboration with researchers from Ruđer Bošković Institute (Ivana Leščić Ašler) and TU Wien (Victor U. Weiss and Martina Marchetti‑Deschmann) have published the scientific publication Exploring the manganese‑dependent interaction between a transcription factor and its corresponding DNA: insights from gas‑phase electrophoresis on a nES GEMMA instrument in journal Analytical and Bioanalytical Chemistry (IF = 3.8).
The publication is focused on the investigation of interactions between manganese metalosensors, specifically the transcription factors MntR from the bacteria Bacillus subtilis and Mycobacterium tuberculosis, and their corresponding DNA sequences. These systems were primarily studied using gas-phase electrophoresis applying a nano electrospray gas-phase electrophoretic mobility molecular analyzer (nES GEMMA) and the obtained results were compared to those of classical EMSA technique. This is the first time that this technique is applied in the study of protein-DNA interactions, so special emphasis was put on the advantages and disadvantages of GEMMA. The obtained results contribute to the understanding of the mechanisms of interactions between DNA and transcription factors that reulate manganese homeostasis in bacteria.
The published results were obtained as part of research conducted within the Croatian Science Foundation project "Manganese Metallosensors" (IP-2020-02-3446) and bilateral project between Austria and Croatia (Native mass spectrometry in analysis of transcription factors modus operandi - Interactions of manganese metallosensors with Mn2+ and DNA).
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